Use of Novel, Rapid Enzyme Based Assays to detect the presence of Sulfate-Reducing Bacteria in Oil and Gas Produced Water (C2026-00198)

Anna Confer, James Fajt, Keith mcLeroy, Kevin Paul Ramirez

Biocides are commonly used to manage bacteria populations on oil and gas production facilities. Each biocide active ingredient, formulation, mode of application, and dose rate will produce different effects on bacteria populations. The only way to accurately assess the effect of biocides is to conduct bacteria population testing. Sulfate reducing bacteria (SRB) are known to sour production and contribute to microbial induced corrosion. For those reasons, oil and gas production operators are routinely conducting testing to assess SRB populations. To manage biocide application and assess the risk, there is a need to know if SRB are present and total bacteria load. This paper reports on the design, application and interpretation of two separate enzyme-based assays. One assay determines total bacteria load, while the other detects the presence of SRB. The assays react with bacteria enzymes, producing a dye that is proportional to the magnitude of the bacteria population. The assays produce results in one day, are compact, portable, use non-hazardous, non-toxic supplies, and do not require incubation or refrigeration. By combining these results, an in-depth bacteria risk assessment can be conducted. The assays can also be used before and after biocide application to determine if treatment meets bacteria management goals.